Bad Boys II Movie Review

Bad Boys II Movie Review The film Bad Boys II, starring two cops named Marcus and Mike (Martin Lawrence and Will Smith), is set in Miami where both partners are assigned to look for clues and evidence for an international drug dealer’s arrest. As they start with the investigation and stake outs, they find themselves insulting each other, fighting like little brothers, and messing up operations. Aside from cop work, Marcus soon discovers some information about the whereabouts and actions of his sister (Gabrielle Union), who is also an undercover agent working for the FBI. Stunned, Marcus becomes irritated with his partner and debates if he really should transfer. The movie’s genre was mostly action-packed. The strengths of the action part because the actors were racing against time to achieve a goal, the shootouts that took place, and tons of explosions. The strength of the romance parts shows Mike and Marcus’ sister together occasionally showing their love affections to one another while trying to hide the secret from Marcus. The strength of the comedy part is when the partners argue or when Marcus freaks out about his sister dating his Mike. A weakness throughout the entire movie was there was too many arguments between the partners when the focus of the movie should’ve been on the case. The movie fulfilled all the parts of story plot, but the visual effects and the audio needed work. One problem was that during the night scenes, the lighting throughout the scene wasn’t good because I couldn’t tell who was talking, who the characters were, and where the cameras were supposed to be focused in on. Naturally, actors’ voices tend to be quieter at night to fit the mood in the scene but I could barely hear the characters’ lines. There were no big distractions that deterred my attention away from the focus of the scene, it’s just the work of the audio and visual. This could’ve been fixed by adding mics on the actors adding props like light posts to or candles near them to enhance the lighting in the scene. While the movie is filled with action and is barely slowed paced, certain parts are realistic but not all. For example, when the international drug dealer, Carlos ‘Johnny’ Tapia’s (Jordi Molla) operation is discovered by the cops, he decides to takes Marcus’ sister hostage after discovering she is an agent. In my opinion, this isn’t realistic because you wouldn’t have your sister working as an agent, she wouldn’t be working on the same mission as you, and she wouldn’t get kidnapped to be forced to leave to Cuba with the enemy. Another example is during a chasing scene, Mike is behind the wheel, Marcus and his sister are trying to lose the enemies in pursuit by shooting at them. At one point they cross through a village where there looks to be inhabited by Cubans but there is no people in the huts or around the village. If there are two trucks running through a village, destroying their property, where was everyone? Where did they go? The least the movie directors could’ve done is have people scream in fear to make it look inhabited. Compared to the first â€Å"Bad Boys† movie, this movie is completely differently but does have the same story plot. In the first movie, there is a drug dealer or the antagonist, the two heroes, the sidekick, and operations. The second movie has everything the first movie has just a little more detailed than the first. The difference between the two movies was the time difference. The time was really important when comparing the movies together, because of the advancement in the camera technology, the filters used, and how realistic the explosions were or the actors’ lines. Overall, I believe the movie is an ok movie. It’s a film worth seeing if you’re into action, and like explosions spraying across the television. Aside from being action-packed and funny, it can also be informative, which is something not a lot of movies directors input into movies nowadays. Michael Bay has proven to world yet again that this film can be a hyperkinetic. Bay gives the audience a new surprise around every corner, giving the movie a new kind of excitement. Some of the viewers and fans have also requested a third movie to come out and directors have heard requests and plan on making a sequel, meaning this won’t be the last time hearing from the two bad boys for life.

The security properties of network applications Assignment

The security properties of network applications - Assignment Example That is; confidentiality, integrity and availability of data to the right person. Not only will this report cover the network applications security, but it will also look into the network applications architecture and how this architecture can be best implemented so as to ensure data confidentiality, data integrity and data availability. For this case study, BF is a social network application that aids individuals to have a social forum with other people. Through that, an interested individual will have to register with BF so that they can be in a position to communicate with other individuals through the use of text and sending of images and videos. 1.2 Application description What are network applications? These are programs that run on a network and they allow for more than one user to have access to the program. Network applications can either be run online or offline. Offline network applications can be related to programs such as Office. These programs can be installed and/ or networked from the main server so that they can allow more that one individual to have access to using the program. When it comes to online network applications, these are applications that need an online server and a client; both of which must communicate in real time else they will no longer be referred to as online network applications. In this case, there is a server which is located in the data centre. The server stores all the relevant data and information that may be queried by a client. The client on most occasions could be a web browser or another computer/ laptop/ or gadget. How does BF work? What is the user experience of BF? Based on the above application description, the same ideology can be applied to our case; that is the BF social network application program. It is important to note that BF also uses the same application description where there exists a server and a client. The servers (s) are always located in the data centre. This is to ensure that they are not acc essed by individuals who are not authorised to come into contact with them. In addition to that, the client in this instance will be the web browser that the user will use so as to key in the Universal Resource Locator (URL) of BF. Example: http: //www.BF.com Once the user hits the search button from their web browser, that will send a request for the search of the URL (http: //www.BF.com) and the corresponding BF server will respond to the request by providing that particular web page that the user searched for. After that, the user will register/ sign up with BF by providing their personal details such as: their email address, physical address and real names. Assumptions made about BF: Based on the BF social network application program, some of the assumptions that can be made include: Communication concurrency: Due to the fact that BF is an online social network application, information is prone to be exchanged between the server and the client. With that, communication concurren cy should be adhered to strictly. An assumption is that the lack of communication concurrency between the server and the client will lead to data loss. Stability: Stability is critical to a social network application program. Lack of stability can cause huge losses due to downtime of the program. This is majorly caused when the servers cannot handle user’s overwhelming requests. Data security: There is exchange of data between the server and the client. Therefore, data security should be critically analysed. An assumption is

Mental health Essay Example | Topics and Well Written Essays - 750 words

Mental health - Essay Example Due to this broad concept of mental health there have been a set of studies but a number of writers out of which some are supporting each other and some are contradictory. Mental health has been studies in the different form it gets manifested and with different factors that either directly or indirectly influence mental health. These studies have focused on different influential factors from the society, personal, and cultural components of a living being. We will be focusing on the work done by four writers Pedersen, Patterson, Weinrach, and Speight. All these writers have written a number of articles which are presenting different views. Weinrach and speight say â€Å"Although racial discrimination exists, both within and outside of the counseling context, the Competencies do little to combat it. In fact, the Competencies actually promote viewing persons primarily as members of specific racial and ethnic groups. The Competencies exist at a symbolic and an applied level. Significa nt problems exist for mental health counselors at both of these levels. The Competencies greatest flaw is their preoccupation with perceived deficits in clients, the counseling profession, and American society. It is virtually impossible to separate the content of the Competencies from the political process that has surrounded efforts to promote their universal adoption† (Weinrach and Thomass 2004). Most of their work is focused on multicultural counseling and mental development and also the impact of one’s competencies and mental development secondary to that. This in conclusion means that culture has a major role to play in mental development though competencies might do the same but not as efficiently as multicultural counseling. â€Å"At the macro level, individuals and families are the target and the mental health practitioners derive their

Analysis of Fallen by Jane Hammond Research Paper

Analysis of Fallen by Jane Hammond - Research Paper Example Art can only be appreciated relatively on an individual perspective. The influence and importance of art can be seen by their presentation of society. The ancient art can be appreciated when interest and urge to understand the past and their role in the society are evaluated (Barnet 89). Several writings in the English language have been employed in the development of the artistic impressions that create a powerful artistic appearance. With the increasing space utilization and the need for information transfer, the work of art displayed on the Great Wall of Los Angeles is a combination of both art and information transfer. Fallen by Jane Hammond Jane Hammond artwork is based on experience in Iraq. The Iraq war had a massive impact on the works of artists. Fallen by Hammond is a work of art focusing on the problems of the soldiers. The soldiers lost lives in the war, and the work of art by Jane Hammond is based on remembering the fallen heroes of the Iraq war. The art is essential is helping the society realize the beauty of memories and the impact of the war in society. However, making an artwork that covers issues of war without raising emotions is hard because of the emotive nature of the issues. The art by Hammond is effective in creating a memoir of the fallen heroes. The art involved using a collection of leaves that are relevant (Eaves 67). It is a non commissioned memorial to the fallen heroes of the Iraq war.   The created paper leaves are placed on a horizontal platform. The fallen artwork departs from the conventional practice of using bronze and durable materials. The number of leaves on the platform increases as the length of the pedestal is increased leading to the creation so a wider pedestal. The use of the fallen leaf symbolizes the end of life making the memorial effective and easy use (Eaves 56).  

Chromium Induced Toxicity Research

Chromium Induced Toxicity Research Abstract In the present study, we hypothesize that cytotoxicity, genotoxicity and oxidative stress play a key role in chromium induced toxicity in SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines when exposed for 24 h. Acute toxicity tests were conducted on three fish species namely L. calcarifer, E. suratensis and C. catla by exposing them to different concentration (0, 10, 20, 30, 40 and 50 mg/L) of chromium for 96 h under static conditions and the LC50 was calculated. The percentage cell survival was assessed by multiple endpoints such as MTT, NR, AB and CB assays were performed in seven fish cell lines exposed to different concentrations of chromium and EC50 values of all the four endpoints was calculated. Linear correlations between each in vitro cytotoxicity assay and the in vivo mortality data were highly significant. Microscopic examination of cell morphology indicated cell shrinkage, cell detachment, vacuolations and cell swelling at highest concentration of chromium (50mg/L). The DNA damage and nuclear fragmentation were assessed by comet assay and Hoechst staining, in seven fish lines exposed to different concentrations of chromium. The result of antioxidant parameter obtained show significantly decreased catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GSH) and Glutathione peroxidase (GPx), and increased level of lipid peroxidation (LPO) in all the cell lines after exposure to increasing chromium in a concentration-dependent manner. This results proves that fish cell lines could be used as an alternative to whole fish using cytotoxicity, genotoxicity and oxidative stress assessment after exposure to chromium. Keywords: Fish cell lines, Chromium, Cytotoxicity, Genotoxicity, Oxidative stress 1. Introduction Heavy metal pollution of water is a serious environmental problem facing the modern world. At global level heavy metals pollution is increasing in the environment due to increase in number of industries (Chidambaram et al. 2009). Industrial effluents are discharged into the sewage canals, rivers and irrigation water, causing major pollution and health hazards (Baddesha and Rao 1986). Many industrial wastewaters contain heavy metals like cadmium, lead, zinc, cobalt and chromium. The toxic heavy metals are mostly absorbed and get accumulated in various plant parts as free metals which may adversely affect the plant growth and metabolism (Barman and Lal 1994). Human beings and cattle are badly affected when these metals are incorporated into food chain as it causes bronchitis and cancer (Khasim et al. 1989; McGrath and Smith 1990; Nath et al. 2005). Among heavy metals, chromium plays a major role in polluting our aquatic environment system. In nature chromium occurs predominately in two valances Cr (III) and Cr (VI). Hexavalent chromium [Cr (VI)] predominates over the Cr (III) form in natural waters. Hexavalent chromium [Cr (VI)] particulates enter the aquatic medium through effluents discharged from leather tanning, textiles, chrome electroplating, metal finishing, dyeing and printing industries and several other industries. The Cr (VI) penetrates biological membranes easily and causes cellular damage by oxidative stress (Irwin et al. 1997; Begum et al. 2006), its unselective exposure may pose serious effect on aquatic communities including fish. Toxic effects of Cr(VI) on enzymological/biochemical (Al-Akel and Shamsi 1996; Vutukuru et al. 2007; Oner et al. 2008), hematological (Gautam and Gupta 1989; Al-Akel and Shamsi 1996), immunological (Prabakaran et al. 2007) parameters, endocrine toxicity (Mishra and Mohanty 2009) and genotoxicity (Chen et al. 2011) have been reported in many teleosts fishes. In environmental risk assessment, much of the toxicity test on fish has involved the use of lethality as the endpoint. On the other hand, in vivo bioassay is expensive and requires huge quantity of toxicant. The exposure time is only 24 h as opposed 96 h in bioassay, which could reduce the cost of labor, lab facilities and test time but more importantly allow decisions to be made more rapidly. Nevertheless, toxicity testing with fish is an essential part of environmental risk assessment procedures (Castano et al. 2003). For all these considerations, the development and use of in vitro assays that could measure early stages of toxicity in vertebrates represent an approach that could be very useful to monitoring environmental risk assessment (Walker 1999). Over the last four decades, cell and tissue culture methods have been refined and have now become an essential tool in environmental research. There are a lot of ethical, scientific and economical reasons that support the development of in vitro methods for use in ecotoxicology (Castano and Gomez-Lechon 2005; Bols et al. 2005; Schirmer, 2006; Fent 2007; Taju et al. 2012, 2013, 2014). The use of fish cell lines in environmental toxicology has been reviewed and positively assessed mainly with regards to cytotoxicity (Babich and Borenfreund 1991; Castano et al. 2003; Fent 2001). Cytotoxicity assessments can be readily employed to examine multiple endpoints, including measurements of cell death (apoptosis), cell viability, cellular morphology, cell metabolism, cell attachment/detachment, cell membrane permeability, proliferation, growth kinetics, genotoxicity and oxidative stress (Maracine and Segner 1998; Li and Zhang 2002; Shuilleabhain et al. 2004; Taju et al. 2014). In the present study, three fish species from three different aquatic environments, Lates calcarifer (Marine), Etroplus suratensis (Brackishwater) and Catla catla (freshwater) were selected as representatives of their respective environments to study their suitability for acute toxicity test to evaluate the potential risk of chromium (Cr). They are excellent food fishes with a good market demand in India, Malaysia, Bangladesh and Pakistan. Some attempts were made to study in vivo acute toxicity in Sea bass, Etroplus and Catla using various toxicants (Chezhian et al. 2010; Azmat and Javed 2011, 2012; Bhat et al. 2012; Taju et al. 2012, 2013). The seven fish cell lines namely SISK and SISS cell lines derived from L. calcarifer (Sahul Hameed et al. 2006; Parameshwaran et al. 2006b), SICH and ICG cell lines derived from C. catla (Ishaq Ahmed et al. 2009b; Taju et al. 2014), and IEE, IEK and IEG cell lines derived from E. suratensis (Sarath Babu et al. 2012) were used as in vitro assays t o evaluate the cytotoxicity, genotoxicity and oxidative stress exposed to chromium. The results of in vitro cytotoxicity were compared with the results of in vivo acute toxicity test using fish. The use of these cell lines for toxicity assessment of chromium instead of living fish is recommended. 2. Material and methods 2.1. Chemicals and reagents Tissue culture media and chemicals were obtained from GIBCO (Invitrogen Corporation, USA). Potassium dichromate (K2Cr2O7), EDTA, Trichloroacetic acid, DTNB [5,5-dithio-bis-(2-nitrobenzoic acid)], Thiobarbituric acid, Hydrogen peroxide, Nitro blue tetrazolium (NBT), Riboflavin, Hydroxylamine-HCl, Triton X-100, Ethidium bromide, Methanol, Acetic acid, Sodium chloride, Sodium hydroxide and Coomassie Blue was purchased from SRL chemicals, India. 2.2. Collection of experimental animals Lates calcarifer and Etroplus suratensis were collected from Central Institute of Brackishwater Aquaculture (CIBA), Chennai. Catla catla was collected from a local pond in Walajapet, Vellore District, Tamil Nadu, India. The experimental fishes were 2 3 g in body weight. Specimens were transported live in oxygen bags or buckets to the laboratory, acclimatized and maintained for 20-30 days in a salinity range of 5-10 ppt for E. suratensis, 20-25 ppt for L. calcarifer and in freshwater in the case of C. catla (23-28oC) under an ambient photoperiod in the laboratory for 10 days prior to experiments. The fish were fed with commercial pellet feed twice a day and starved for 24 h before and during the experiments. 2.3. In vivo fish acute toxicity test Fish acute toxicity tests were conducted by exposing E. suratensis, L. calcarifer and C. catla (N = 10 per aquarium) for 96 h to chromium under static conditions (OECD 203, 1992). Five different concentrations chromium i.e., 0, 10, 20, 30, 40 and 50 mg/L diluted with seawater (5 ppt) and freshwater while control with sea water and freshwater alone were tested to determine the LC50 (concentration at which 50% of the fish population dies). The aquaria had a working volume of 30 lit based on the body weight of fishes (1 g fish/L). Dead fishes were counted and removed immediately every day. All the experiments were conducted in triplicates. Mortalities were recorded following the guideline for fish acute toxicity OECD 203 (1992). 2.4. Fish Cell lines A total of seven cell lines established from different organs of L. calcarifer (SISS-seabass spleen, SISK-kidney), E. suratensis (IEE Etroplus eye, IEG gill, IEK kidney) and C. catla (SICH Catla heart, ICG gill) were tested for their sensitivities to chromium. These fish cell lines were propagated at 28oC in Leibovitzs L-15 medium (pH 7.0 -7.4) with 2mM L-glutamine, 10% foetal bovine serum (FBS), penicillin 100 IU/ml and streptomycin 100 ÂÂ µg/ml. The osmolarity ranged from 300 to 360 mOsm kg-1. These cells were sub-cultured every 2-3 days using standard procedure. Cells at exponential growth phase were harvested and used for in vitro cytotoxicity tests. 2.5. In vitro cytotoxicity assay using fish-derived cell lines SISS, SISK, IEE, IEK, IEG, SICH and ICG cells at exponential growth phase were collected and diluted to a concentration of 105 cells/ml in Leibovitzs L-15 medium with 10% FBS. After agitation, the cells were added to each well of 96-well tissue culture plates at the concentration of 2 x 104/well and incubated overnight at 28oC. After incubation, the medium was removed and the cells were re-fed with medium containing 0 (control), 10, 20, 30, 40 and 50 mg/L of chromium for 24 h EC50 analysis. Then four endpoints for cytotoxicity, i.e., MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] assay, Neutral red (NR) uptake assay, Alamar blue assay (AB) and protein concentration for Coomassie blue (CB) assay were determined after 24 h exposure as described by Borenfreund et al. (1988), Borenfreund and Puerner (1985), Taju et al. (2012) and Shopsis and Eng (1985), respectively. 2.5.1. Cell morphology SISS, SISSK, IEE, IEK, IEG, SICH and ICG cells were plated into a 24 well tissue culture plate at a density of 2ÃÆ'-105 cells (in 1 mL growth medium). After overnight growth, supernatants from the culture plates were removed and fresh aliquots of growth medium containing various concentrations of the chromium (0, 10, 20, 30, 40 and 50 mg/L) were exposed for 24 h. Upon incubation, cells were washed with phosphate-buffered saline (PBS, pH 7.4) and the morphological changes were observed under an inverted phase-contrast microscope (Carl Zeiss, Germany) at 100ÃÆ'- magnification. 2.6. Assessment of in vitro genotoxicity using fish-derived cell lines 2.6.1. Comet assay The Single Cell Gel Electrophoresis (comet assay) was performed on SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines according to the method of Singh et al. (1988) with slight modifications in accordance with the protocols of Taju et al. (2014). 5 x 104 cells on 500 ÃŽÂ ¼L of complete culture medium were seeded per well in a 24-well-plate. After 24 h incubation, cells were exposed to chromium using the following concentrations: 0 (control), 10, 20, 30, 40, 50 and 60 mg/L. At the end of the exposure period, cells were collected through trypsinization, followed by centrifugation at 1000 rpm for two minutes to obtain the pellet and avoid cell loss. After the centrifugations, the supernatant was discarded and the pellet resuspended in 100 ÃŽÂ ¼L of 0.9% agarose in milliQ water (low-melting point agarose, Sigma Aldrich chemicals, USA). The suspensions of cells in agarose were then applied dropwise to microscope slides containing an agarose layer (agarose electrophoresis grade, prep ared with a 1% concentration in milliQ water), and kept in a freezer for 10 min. The cells were lysed in freshly made lysing solution (2.5 M NaCl, 100 mM EDTA, 10 mM Tris-HCl, 10% DMSO, 1% Triton X-100, pH 10), for 1 h at 4 ÂÂ °C. After rinsing with redistilled water, the slides were placed on the horizontal gel box, covered with the cold alkaline buffer (0.3 M NaOH, 1 mM EDTA, pH >13) and left for 20 min. Electrophoresis was run in the same buffer at 25 V (0.83 V/cm) at 300 mA for 20 min at 4 ÂÂ °C. After electrophoresis the slides were neutralized in a cold neutralization buffer (0.4 M Tris-HCl, pH 7.5), for 2 to 5 min, fixed in methanol:acetic acid (3:1) for 5 min and stored in the dark at room temperature. Prior to examination, the slides were rehydrated and stained with 10 ÂÂ µg/mL ethidium bromide and examined using a Zeiss Axioplan epifluorescence microscope (Carl Zeiss, Germany). A positive control (5 ÂÂ µM H2O2) was also included in every batch of sample s. This strategy was chosen to compare the variation in the distance of migration. The positive control was not included in evaluation. Slides were examined at 100x magnifications using a fluorescence microscope. For each experimental condition 100 randomly chosen cells from two duplicate slides were examined (50 from each slide). In all 100 comets were scored visually according to the relative intensity of the fluorescence in the tail length. The extent of DNA migration was determined as a percentage of DNA in the tail (% tDNA) using an image analysis system comet 5, Kinetic Imaging Ltd. 2.6.2. Assessment Nuclear fragmentation by Hoechst 33258 Nuclear fragmentation of SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines was analyzed with Hoechst 33258. The cells were seeded in 12-well cell culture plates and incubated overnight. Then the cells were treated with different concentrations of chromium (0, 10, 20, 30, 40 and 50 mg/L). Cells were fixed in 4% paraformaldehyde in PBS for 30 min, washed with PBS, and stained with 1 ÃŽÂ ¼g/mL Hoechst 33258 in PBS for 30 min. Stained cells were washed twice with PBS. The changes in nuclei were observed with a fluorescent microscope through a UV filter. 2.7. Preparation of cell extract and Biochemical estimations The SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines were exposed to different concentrations of chromium (0, 10, 20, 30, 40, 50 and 60 mg/L) on 25 cm2 flasks for 24 h. After 24 h they were trypsinized and pelleted by centrifugation at 500ÃÆ'-g for 5 min. The cell pellet was washed with PBS (0.1M, pH7.4), resuspended in 500 ÂÂ µl chilled homogenizing buffer (250mM sucrose, 12mM Tris-HCl, 0.1mM DTT, pH 7.4) and lysed using Dounce homogenizer. The lysate was centrifuged (8000ÃÆ'-g, 10 min, 4 ÂÂ °C) and the supernatant (cell extract) was used for various biochemical assays. Protein concentration in the cell extract was estimated by the method of Lowry et al. (1951). The enzymatic antioxidant superoxide dismutase (SOD) activities were determined by following the procedures described by Kono (1978). Catalase (CAT) activity was determined by following the method described by Aebi (1974). The level of non-enzymatic antioxidant reduced glutathione (GSH) was estimated following the procedures described Saldak and Lindsay (1968). The activity of glutathione peroxidase (GPx) was assayed by the method of Flohe and Gunzler, (1984). The level of lipid peroxidation (LPO) was measured according to the method described Beuge and Aust (1978) based on the reaction with thiobarbituric acid. The results were recorded as ÂÂ µmol of TBA reactive substances/mg protein. The enzymatic and non-enzymatic parameters was expressed as ÂÂ µmol/mg protein. 2.8. Data analysis Experiments were performed in triplicate with eight replicates for each exposure concentration. Absolute values of each assay were transformed to control percentages. The results of LC50 and EC50 values were expressed as dilution in (mg/L) of the sample calculated using computerized (EPA, 2000) software. The individual data points of the concentration response cytotoxicity graph were presented as the arithmetic mean percent inhibition relative to the control standard error (SE). Cell viability and the concentration were fitted Scatter plots with the regressive equation (a linear regression model). The strength of the r2 value was used to determine whether a linear or quadratic relationship was assumed. Analysis of variance was used to determine whether groups of variables differed from each other (SPSS, Version 16). 3. Results The cumulative percentage mortality in L. calcarifer, E. suratensis and C. catla exposed to different concentrations of chromium was determined at 96 h and the results are presented in Fig 1. The toxic effect of chromium on the survival of fish was found to be concentration and time dependent. The chromium at the concentration of 50 mg/L caused 100%, 96.66% and 90% mortality, respectively, in L. calcarifer, E. suratensis and C. catla, whereas lower concentration of chromium at 10 mg/L caused 26.66%, 16.66% and 20% mortality of L. calcarifer, E. suratensis and C. catla respectively. No mortality was recorded in the control fish even after 96 h exposure. The LC50 values corresponding to 24, 48, 72 and 96 h of exposure of chromium were determined and results are presented in Table 1. Five different concentrations which ranged from 10 to 50 mg/L of chromium were used to carry out the in vitro toxicity assay in SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines using four cytotoxicity end points (MTT, NR, AB and CB assays) and the results are shown in Fig.2 A-D. The cytotoxicity of chromium to SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines was found to be similar in all the toxic endpoints employed. The lowest concentration of chromium tested (10 mg/L) was found to toxic in all the cell lines particularly SICH and IEK cell lines. The progressive increase in the concentration of chromium led to increase in toxicity when compared to control cells. The MTT, NR, AB and CB cytotoxicity endpoint assays revealed that a 24-h exposure of all the cell lines to different concentrations of chromium produced a dose-dependent reduction in the fraction of viability. The EC50 values and 95% confidence limit values obtained for chromium are summarized in Table 2. Correlations a mong the endpoints employed in the SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines to study cytotoxicity of chromium have been determined. A general tendency in the sensitivity among the four endpoints could be observed and statistical analysis revealed good correlation with R2 = 0.889-0.927 for all combinations between endpoints (Data not shown). The in vivo values of L. calcarifer vs. in vitro data of its two cell lines exposed to chromium were highly significant p2=0.956 (L. calcarifer vs. SISS) and 0.962 (L. calcarifer vs. SISK); R2=0.973 and 0.993; R2=0.980 and 0.975; R2=0.992 and 0.977 for MTT (Fig 3A), NR (Fig 3B), AB(Fig 3C) and CB (Fig 3D), respectively. The in vivo values of E. suratensis were compared with in vitro values of its three cell lines (IEE, IEG and IEK) exposed to chromium and were found to be highly significant p2=0.985 (E. suratensis vs. IEE), 0.987 (E. suratensis vs. IEK) and 0.968 (E. suratensis vs. IEG); R2=0.980, 0.936 and 0.956; R2=0.961, 0.955 and 0.904 and R2=0.955, 0.939 and 0.974 for MTT (Fig 3E), NR (Fig 3F), AB(Fig 3G) and CB (Fig 3H), respectively. Linear correlations between each in vitro vs. in vivo (C. catla)values of chromium were highly significant p2=0.991 (C. catla vs, SICH) and 0.993 (C. catla vs, ICG); R2=0.982 and 0.983; 0.974 and 0.990 and 0.987 and 0.984 for MTT (Fig 3I), NR (Fig 3J), AB(Fig 3K) and CB (Fig 3L), respectively. The prominent morphological changes of the cells exposed to high concentrations of chromium were observed. The changes observed include cell shrinkage, cell detachment, vacuolations and cell swelling in SISS (Fig 4H), SISK (Fig 4I), IEE (Fig 4J), IEK (Fig 4L), IEG (Fig 4L), SICH (Fig 4M) and ICG (Fig 4N) cell lines. In controls, no morphological alterations were observed in the SISS (Fig 4A), SISK (Fig 4B), IEE (Fig 4C), IEK (Fig 4D), IEG (Fig 4E), SICH (Fig 4F) and ICG (Fig 4G) cell lines. The percentage of DNA damage and the cumulative tail length from 100 cells per sample were measured in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells exposed to different concentrations of chromium (0, 10, 20, 30, 40 and 50 mg/L) and the results are shown in Fig. 5. The length of tail DNA in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells exposed to 10 mg/L of chromium was estimated to be about 1.7%, 2.0%, 1.3%, 1.5%, 2.1%, 1.4% and 1.5%, respectively at a 24-h exposure, and chromium at the concentration of 50 mg/L caused 8.9%, 11.0%, 9.4%, 8.8%, 11.1%, 6.4% and 7.2% of tail DNA migration in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells, respectively (Fig. 5). Comet results of chromium exposed SISS, SISK, IEE, IEK, IEG, SICH and ICG cells showed a dose dependent increase in tail DNA (%) compared to the control cells, which gave the extent of DNA damage. The SISS, SISK, IEE, IEK, IEG, SICH and ICG cells were exposed to chromium for 24 h at different concentrations (0, 10, 20, 30, 40 and 50 mg/L) and the results are shown in Fig. 6A-N. Apoptotic cells were identified by Hoechst staining of condensation and fragmentation of the nuclei as shown in SISS cells (Fig. 6H), SISK cells (Fig. 6I), IEE cells (Fig. 6J), IEK cells (Fig. 6K), IEG cells (Fig. 6L), SICH cells (Fig. 6M) and ICG cells (Fig. 6N) at higher concentration i.e. 50 mg/L of chromium exposed for 24 h, while no nuclear changes were observed in control cells are shown in SISS cells (Fig. 6A), SISK cells (Fig. 6B), IEE cells (Fig. 6C), IEK cells (Fig. 6D), IEG cells (Fig. 6E), SICH cells (Fig. 6F)and ICG cells (Fig. 6G). The level of antioxidant parameters such as SOD, CAT, GPx, GSH and LPO was measured in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells exposed to different concentrations of chromium and the results were presented in Fig 7A-E. Regarding oxidative stress biomarkers, no significant change was observed in SOD, CAT, GSH and LPO levels in the SISS, SISK, IEE, IEK, IEG, SICH and ICG cells exposed to lower concentrations i.e. 10 mg/L of chromium when compared to the control cells. However, when these cell lines were exposed to 50 mg/L of chromium, the activity of SOD (~2.1, ~2.3, ~1.5, ~1.3, ~2.3, ~1.2 and ~2.2 fold in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells respectively in Fig 7A), CAT (~5.2, ~6.8, ~5.3, ~7.4, ~6.4, ~5.2 and ~4.6 fold in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells respectively Fig 7B) and level GSH (~1.6, ~1.5, ~1.3, ~1.6, ~1.5, ~1.8 and ~1.3 fold in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells respectively Fig 7C) and GPx (~1.2, ~1.1, ~1.0, ~1.2, ~1.1, ~0.9 and ~1. 3 fold in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells respectively Fig 7D) decreased was found to be significantly (*P 4. Discussion Heavy metals constitute a main group of aquatic pollutants due to their bioacuumulative and non-biodegradable properties (Velma and Tchounwou 2010). Their excessive contamination of aquatic ecosystems has evoked major environmental and health concerns worldwide (Vutukuru et al. 2007). Chromium is the sixth most abundant heavy metal in the earth crust (U.S. EPA 1984). Fish and Fish cell lines constitute an excellent model to understand the mechanistic aspects of metal toxicity (Taju et al. 2014). In this study, we have examined the in vivo toxicity in three fish species in different environment i.e. L. calcarifer (Marine water), E. suratensis (brackish water) and C. catla (Fresh water), and in vitro cytotoxicity, oxidative stress and genotoxicity of the three same fish cell lines, SISS, SISK (Seabass spleen and kidney cell lines), IEE, IEK, IEG (Etroplus eye, kidney and gill cell lines), SICH and ICG (Catla heart and gill cell lines) an exposure to chromium. The results of this study clearly show that the fish cell lines experienced oxidative stress by modulating the antioxidant enzyme, exhibited DNA damage, nuclear fragmentation and microscopic morphological changes in the SISS, SISK, IEE, IEK, IEG, SICH and ICG cells. The LC50 values of chromium were determined as 30.22, 33.83 and 30.64 mg/L respectively in L. calcarifer, E. suratensis and C. catla, respectively at 96 h of exposure in this study. Recently, Mishra and Mohanty (2009) reported the LC50 values of chromium on Channa punctatus at 96 h of exposure as 41.75 mg/L. The LC50 values observed by Mishra and Mohanty (2009) were found to be higher when compared to L. calcarifer, E. suratensis and C. catla and this indicates that the L. calcarifer, E. suratensis and C. catla were found to more sensitive to chromium. Seven fish cell lines derived from L. calcarifer (SISS SISK), E. suratensis (IEE, IEK and IEG) and C. catla (SICH and ICG) were applied to evaluate the cytotoxicity of chromium using MTT, AB, NR and cell protein (CB) assays. The results of in vitro assays were compared with the results of in vivo test to determine the suitability of these fish cell lines for toxicological studies to replace the use of whole fish. The evaluation of cytotoxicity of chemical substances using animal cells has been carried out by many workers (Ekwall 1980a, 1983; Metcalfe 1971; Muir 1983a, 1983b; Paganuzzi et al. 1981; Benoit et al. 1987). Four commonly used endpoint assays (MTT, NR AB and cell protein assay CB) were employed in the present study using SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines of E. suratensi, C. catla and L. calcarifer to evaluate the in vitro cytotoxicity of chromium. The main observation was that the cytotoxicity was closely associated in all the seven cell lines independent of the toxic endpoints employed. This not only supports the observations of Ekwall (1995) and Li and Zhang (2002) that most cell lines have a similar results to toxicants when toxicity is measured by different endpoints, corresponding to inhibition or destruction of basal functions and structures, and also suggests that endpoints employed in the present study can also be used to predict acute cytotoxicity. Tan et al. (2008) have used six fish cell lines to study the toxicity of four heavy metals: cadmium, chromium, zinc, and copper by using two cytotoxicity endpoints MTT and CB assays. The results revealed that carp epithelioma cells are least tolerant to chromium. The NR uptake assay is a useful method for comparing the relative acute cytotoxicity of metals in vitro with metal and chemicals toxicity studies in whole fish in vivo (Brandao et al. 1992; Ryan and Hightower 1994; Taju et al. 2013). In the present study, we employed that SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines for cytotoxicity assessment of chromium by using four endpoints. Our results show that there is no significant difference between all the four endpoints. Segner (1994) reported that the relationship of the in vitro cytotoxicity values to in vivo fish toxicity data is less satisfying and that this might be due to the inconsistency of the in vivo values. As observed in the present study, a positive relationship of acute lethal potency in fish with in vitro cytotoxicity has been found by Fry et al. (1990). Castano et al. (1996) found good correlations between in vivo and in vitro for each endpoint and for the cytotoxicity index and suggested the applicability of the RTG-2 cell line as an alternative protocol to estimate the acute toxicity of chemicals on fish without using live animals. The correlation of in vitro cytotoxicity of metals with in vivo toxicity data was evaluated by comparing the 24 h NR50 results of R1 cells to 96 h LC50 data of different fish species. The rvalues (R1 cell line) were 0.64 for the relation between LC50, data of golden ide and bluegill sunfish, 0.58 for golden ide and rainbow trout in soft water, and 0.68 for golden ide and rainbow trout in hard water (Segner et al. 1994). In the present study, in vitro cytotoxicity of chromium with in vivo results was evaluated by comparing the 24 h MTT, NR, AB and CB data of seven Indian fish cell lines to 96 h data of three fish species (L. calcarifer, E. suratensis and C. catla). A good correlation was found between in vitro of seven fish cell lines compared with in vivo values of whole fish exposed to chromium for 24 h and 96 h respectively, with r=0.902 to 0.99. The results revealed that the four endpointsvalues were closely correlated to whole fish in vivo values and that the linear correlation b etween each in vitro parameter and the in vivo data were found to be highly significant. The results of in vitro assays using SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines of E. suratensis, C. catla and L. calcarifer were correlated with those obtained from in vivo assay using the same species of fish (L. calcarifer, E. suratensis and C. catla). Based on the results of the present study we recommend the use of these seven cell lines instead of living fish for toxicity assessment of metal salts and environmental contaminants. The present study showed that chromium induced genotoxicity in SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines by comet assay. DNA damage was observed in SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines exposed to chromium in a concentration dependent manner. The DNA damage at higher test concentrations in SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines could be due to the elevated levels of tail DNA in all cell lines compared to their controls cells. Induction of ROS under metallic stress could attack the DNA and damage its integrity. Our present results are similar to the previous reports (Iqbal Ahmad et al. 2006; Velma and Tchounwou 2010; 2013) DNA damage in gill and kidney of Anguilla anguilla L. exposed to chromium with or without pre-exposure to ÃŽÂ ²-naphthoflavone. In another study, medaka fin cell lines exposed to Cr (VI) to examine the genotoxic potentials, have observed DNA double strand breaks a

Child Abuse in A Child Called It by Dave Pelzer Essay -- A Child Calle

In American society today we fail to address several issues that need to be addressed. Unfortunately, child abuse is one of the major issues that our country is plagued with, yet we neglect to bring this to the attention of the entire nation. It is often over looked because everyone has a different view of what exactly defines child abuse. The International Child Abuse Network (ICAN) uses four basis categories to docunment the child abuse cases. They are: emotional abuse, neglect, physical abuse, and sexual abuse. I will be describing the first three. Emotional Abuse, (also known as: Verbal abuse, mental abuse, and psychological cruelty) includes acts or the failures to act by parents or caretakers that have caused or could cause serious behavioral, cognitive, emotional or mental disorders. This can include parents and/or caretakers using extreme or bizarre forms of punishment, such as the child being confined in a closet or dark room, being tied to a chair for long periods of time, or threatening or terrorizing a young mind. Less severe acts, but no less damaging is overly negative criticism or rejecting treatment, using degrading terms to describe the child, constant victimizing or blaming the child for situations. Neglect (the failure to provide for the child?s basic needs) can be physical, educational, or emotional. Physical neglect can include not providing adequate food, clothing, appropriate medical care, supervision, or proper weather protection (heating or coats) to the child. Educational neglect can include failure to provide appropriate schooling or special educational needs, allowing excessive truancies, to the child. Psychological neglect is the lack of any emotional support and love, never attending to the child, spousal abuse, or drug and alcohol abuse including allowing the child to participate in drug and alcohol use. Physical abuse is to cause or inflict physical injury upon the child. This may include, burning, hitting, punching, shaking, kicking, beating, or otherwise harming a child. The parent or caretaker may claim not to have intended to hurt the child, that the injury was an accident. It may have however, been the result of over-disciplines or physical punishment that is inappropriate to the child?s age. In 1998 NCANDS (National Child Abuse and Neglect Data System) calculated the Fatalities by Maltreatment, Child Abuse... ... Year Published:  2004 3. The Child Welfare League of America. (1999). State Child Welfare Agency Survey. US Bureau of the Census; ?Estimates of the population of state by age, sex, race & Hispanic origin: 1990 to 1999;? published 12/29/99 Administration for Children and Families Fact Sheets and Publications Table of Contents: Child Maltreatment 2000 Chapter 5 4.  Ã‚  Ã‚  Ã‚  Ã‚  The World Wide Web Virtual Library: The Men's Issues Page  Ã‚   Child Abuse and Neglect Statistics from the National Committee to Prevent Child Abuse April, 1994 and 1995 Number of Child Abuse and Neglect Reports Nationwide  ·Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  American Association for Protecting Children (AAPC). (1988) Highlights of Official Child Neglect and Abuse Reporting, 1986. Denver, CO.: American Humane Association.  ·Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ogintz, E. The Littlest Victim. Chicago Tribune, Thursday, October 6, 1988.  ·Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Sedlak, A. (1990) Technical Amendments to the Study Findings--National Incidence and Prevalence of Child Abuse and Neglect: 1988. Rockville, MD: Westat, Inc.  ·Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Wiese, D. and Daro, D. (1995) Current Trends in Child Abuse Reporting and Fatalities: The Results of the 1994 Annual Fifty State Survey. Chicago, IL.: NCPCA. Child Abuse in A Child Called It by Dave Pelzer Essay -- A Child Calle In American society today we fail to address several issues that need to be addressed. Unfortunately, child abuse is one of the major issues that our country is plagued with, yet we neglect to bring this to the attention of the entire nation. It is often over looked because everyone has a different view of what exactly defines child abuse. The International Child Abuse Network (ICAN) uses four basis categories to docunment the child abuse cases. They are: emotional abuse, neglect, physical abuse, and sexual abuse. I will be describing the first three. Emotional Abuse, (also known as: Verbal abuse, mental abuse, and psychological cruelty) includes acts or the failures to act by parents or caretakers that have caused or could cause serious behavioral, cognitive, emotional or mental disorders. This can include parents and/or caretakers using extreme or bizarre forms of punishment, such as the child being confined in a closet or dark room, being tied to a chair for long periods of time, or threatening or terrorizing a young mind. Less severe acts, but no less damaging is overly negative criticism or rejecting treatment, using degrading terms to describe the child, constant victimizing or blaming the child for situations. Neglect (the failure to provide for the child?s basic needs) can be physical, educational, or emotional. Physical neglect can include not providing adequate food, clothing, appropriate medical care, supervision, or proper weather protection (heating or coats) to the child. Educational neglect can include failure to provide appropriate schooling or special educational needs, allowing excessive truancies, to the child. Psychological neglect is the lack of any emotional support and love, never attending to the child, spousal abuse, or drug and alcohol abuse including allowing the child to participate in drug and alcohol use. Physical abuse is to cause or inflict physical injury upon the child. This may include, burning, hitting, punching, shaking, kicking, beating, or otherwise harming a child. The parent or caretaker may claim not to have intended to hurt the child, that the injury was an accident. It may have however, been the result of over-disciplines or physical punishment that is inappropriate to the child?s age. In 1998 NCANDS (National Child Abuse and Neglect Data System) calculated the Fatalities by Maltreatment, Child Abuse... ... Year Published:  2004 3. The Child Welfare League of America. (1999). State Child Welfare Agency Survey. US Bureau of the Census; ?Estimates of the population of state by age, sex, race & Hispanic origin: 1990 to 1999;? published 12/29/99 Administration for Children and Families Fact Sheets and Publications Table of Contents: Child Maltreatment 2000 Chapter 5 4.  Ã‚  Ã‚  Ã‚  Ã‚  The World Wide Web Virtual Library: The Men's Issues Page  Ã‚   Child Abuse and Neglect Statistics from the National Committee to Prevent Child Abuse April, 1994 and 1995 Number of Child Abuse and Neglect Reports Nationwide  ·Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  American Association for Protecting Children (AAPC). (1988) Highlights of Official Child Neglect and Abuse Reporting, 1986. Denver, CO.: American Humane Association.  ·Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ogintz, E. The Littlest Victim. Chicago Tribune, Thursday, October 6, 1988.  ·Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Sedlak, A. (1990) Technical Amendments to the Study Findings--National Incidence and Prevalence of Child Abuse and Neglect: 1988. Rockville, MD: Westat, Inc.  ·Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Wiese, D. and Daro, D. (1995) Current Trends in Child Abuse Reporting and Fatalities: The Results of the 1994 Annual Fifty State Survey. Chicago, IL.: NCPCA.

Discussing the architecture of Michelangelo

Mannerism refers to a time of European art that began around 1520 in Italy, and lasted until around 1580 to 1600, when the Baroque style of art and architecture began to replace it, but it did continue in many forms until the 17th century. The characteristics of Mannerism include artificial qualities that go against the harmonious, natural elements of High Renaissance art, and a great deal of sophistication, complexity and innovation in design.Michelangelo was one of the greatest practitioners of Mannerism for several reasons. Elegance and innovation are two of the primary elements of Mannerism, and Michelangelo certainly practiced both those elements in his art. Some of his greatest architectural and artistic endeavors contain these elements, combined with sophistication in the design and execution of the works such as the Sistine Chapel's ceiling. The paintings on the ceilings have stood the test of time, and retain their beauty, complexity and eloquence even today.In addition, the concept of painting on the ceiling of a wondrous piece of architecture was also one of Michelangelo's innovations, illustrating how he actively participated in the Mannerism movement. In architecture, Michelangelo also excelled as a Mannerist. â€Å"Mannerist architects were no less interested in ancient classical architecture than were their predecessors, but they found other qualities in ancient Roman architecture to exploit. In fact, they often displayed an even greater knowledge of antiquity than did earlier artists† (Italian Mannerism or Late Renaissnce, 2009).Michelangelo's greatest architectural achievements, such as the Laurentian Library in Florence, helped indicate he was a Mannerist by its' obvious breaking of many architectural rules of the time, showing not only its elegance, but its novelty and sophistication, as well. Michelangelo uses classic design in his building, but adds a new way of assembling them throughout the design in novel and unusual motifs. In th e Palazzo Farnese in Rome, Michelangelo used unnatural and manufactured views throughout the building, another trademark of Mannerist buildings. Many architects view Michelangelo as one of the geniuses of the movement.His, â€Å"Medici Chapel in San Lorenzo was executed, in Vasari's opinion, ‘in a style more varied and novel than that of any other master,' and ‘thus all artists are under a great and eternal obligation to Michelangelo, seeing that he broke the fetters and chains that had earlier confined them to the creation of traditional forms† (Italian Mannerism or Late Renaissnce, 2009). Michelangelo knew how to push the envelope in design and execution, and was interested in change, rather than copying other styles, which are also elements of the Mannerist style of architecture.His greatest Mannerist achievement is St. Peter's Basilica in Rome, a massive project that took him over 18 years to design, and was not completed before his death. This beautiful build ing was dominated by a huge dome that would have been incredible had it been completed during Michelangelo's life. Later changes to the building altered the dome and its effect on the overall building design, but it was one of his greatest achievements, and the innovation and spectacular dimensions of the design helped cement Michelangelo as one of the premier Mannerist architects and artists of the day.Mannerism eventually fell out of favor in Europe, and was replaced by other forms of architecture, including the intricate and detailed Baroque, which followed Mannerism. It was one of the greatest epics of Italian architecture and design, led by one of the greatest artists of all time, Michelangelo. Works Cited Italian Mannerism or Late Renaissnce. (2009, January 16). Retrieved from Italian Mannerism: http://www. cartage. org. lb/en/themes/arts/Architec/MannerismArchitecture/ItalianMannerism/ItalianMannerism. htm Janson's History of Art. (2007). Upper Saddle River: Pearson Education , Inc.

Urban Enterprise Zones

Some policies that could promote economic growth are tax rebates, lowering interest rates and developing Urban Enterprise Zones (UEZs). Providing rebates on home purchases, appliances and home upgrades encourages people to spend money. Lower interest rates work to stimulate home sales, credit card loans and investment in the stock market. UEZs offer lower tax rates, encouraging people to shop and spend money.2) Schumpeter's creative destruction describes how long-term economic growth is sustained by innovative entrepreneurs even when established companies are destroyed. This is less likely to occur in less developed countries because there are less entrepreneurs with less available funds than developed countries. This allows older companies, sometimes even monopolies, to maintain their position in the market.3) With reductions in government spending on higher education, the rate of growth in the United States will surely drop. Less government funding means many students will no longe r be able to afford the cost of tuition.This leads to an unskilled workforce and a downturn in economic growth. Less funding could also translate to lower quality in public colleges and universities. Many people rely on these institutions for respected degrees at a lower cost than private schools. This too would lead to less college graduates and again, an unskilled workforce.